Scaffold-like Micro-well Structures Fabricated by Two-photon-absorption Photopolymerization
We demonstrate a novel two-photon-absorption (TPA) microfabrication technique with potential applications in tissue engineering and other biomedical applications. Complex 3-dimension (3D) microstructures can be constructed using this technique with the advantages of being maskless, single step, real 3D structure and high resolution. By use of this technique, we fabricated scaffold-like micro-well structures with sub-micron resolution and high aspect ratio by both raster scanning and pinpoint scanning methods. The linewidth and height of the resulted micro-wells are controlled by an ultraviolet (UV) pre-exposure step. The finest linewidth is 0.28 m and the highest aspect ratio reaches 6.7.