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Impact Factor: 1.211
ISSN Print: 1609-0985
ISSN Online: 2199-4757
Imprint: Springer
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latest updated: 2015 / 07 / 30
In Vitro Evaluation of Cell Loss: Retention and Repopulation on Substrates upon Shear Flow by a Rheometer
Shan-Hui Hsu, 
Chien-An Chen
Abstract
In this work, we utilized a cone and plate type rheometer to assess the adhesive force between bovine carotid arteries endothelial cells and substrate materials. The cell retention and repopulation on these materials were observed in vitro. The results showed that the rheometer was a convenient tool to evaluate adhesive force and cell retention on a biomaterial substrate. On gelatin-coated surface, cells were more resistant to shear flow compared to those on tissue culture polystyrene or biocompatible poly(carbonate)urethane. The newly grown cells on the gelatin substrate after flushing for a period of time became further resistant to shear flow.
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